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The "2005 Workshop of Thymus Organogenesis" was held in Kyoto, in conjunction with the '4th International Symposium of Kyoto T Cell Conference' to be coordinated as a symposium.

Shiran Kaikan, Kyoto University, Kyoto, Japan April 6 - 10, 2005

April 6

15:00 Registration

18:00 Welcome remarks

18:05 - 19:30 Keynote lecture

Thomas Boehm (Max-Planck-Institute of Immunobiology) Genetic dissection of thymus development: Uncharted aspects of its phylogeny and ontogeny

20:00 Reception at Hotel Fujita


April 7

The organogenesis of the thymus, Chair: Graham Anderson

9:00 - 9:30 Georg Hollånder (University of Basel) On the molecular mechanisms of early thymus development

9:30 - 10:00 Andrew Farr (University of Washington) Extrathoracic thymic tissue in the mouse - isnŐt one thymus enough?

10:00 - 10:15 Norimasa Iwanami (University of Tokushima) Medaka mutations affecting thymus organogenesis

10:15 - 10:30 Hisahiro Yoshida (RCAI RIKEN) Screen of immune disease model mice from ENU-induced mutant pool


Thymic epithelial cells, Chair: Georg Hollånder

11:00 - 11:30 Clare Blackburn (University of Edinburgh) Thymic epithelial progenitor cells

11:30 - 12:00 Hans-Reimer Rodewald (University of Ulm) Insights into the role of the DiGeorge syndrome gene Tbx1 for thymus development from nude mouse blastocyst complementation

12:00 - 12:15 Shigeru Koshiba (Sapporo Medical University School of Medicine) p53-related transcription factors modulate intercellular connections of thymic medullary stromal cells through tight junctions

12:15 - 12:30 Ann Chidgey (Monash University) Reversal of thymic involution by sex steroid ablation: a molecular analysis


Epithelial-mesenchymal interactions for thymus development, Chair: Nancy Manley

14:30 - 15:00 Graham Anderson (University of Birmingham) Role of mesenchyme in thymus development and function

15:00 - 15:30 Ellen Richie (University of Texas) Thymus organogenesis in Pax3 sp/sp embryos

15:30 - 15:45 Hidetoshi Yamazaki (Tottori University) Neural crest-derived cells in the murine developing thymus and their potential for differentiation

15:45 - 16:00 Julie Gordon (University of Georgia) The role of BMP signaling in patterning the common thymus/parathyroid primordium


Lympho-stromal interactions for thymus function, Chair: Bruno Kyewski

16:30 - 17:00 Willem van Ewijk (Leiden University) The Notch signalling pathway is involved in thymic crosstalk

17:00 - 17:30 Nancy Manley (University of Georgia) The Foxn1D/D thymic microenvironment supports T cell development from non-canonical progenitors

17:30 - 18:00 Manami Itoi (Meiji University of Oriental Medicine) The thymic perivascular space as a path of hematopoietic progenitor cells and mature T cells between the blood circulation and the thymic parenchyma

18:00 - 18:15 Taishin Akiyama (University of Tokyo) TRAF6 in thymic stroma is essential for self-tolerance

18:15 - 18:30 Daniel Gray (Monash University) The relative contribution of thymic stromal cell subsets to negative selection

18:30 - 18:45 Mitsuru Matsumoto (University of Tokushima) Essential role of I_B kinase _lpha in thymic organogenesis required for the establishment of self-tolerance

18:45 - 19:00 William Jenkinson (University of Birmingham) Investigating the role of thymic mesenchyme in the regulation of thymocyte development


April 8

Dynamics of developing thymocytes, Chair: Howard Petrie

9:00 - 9:30 Howard Petrie (Scripps Florida) T lineage commitment: extrathymic or intrathymic?

9:30 - 10:00 Conrad Bleul (Max-Planck-Institute for Immunobiology) A recent thymic immigrant maps to the branching point of the T versus B lineage decision

10:00 - 10:30 Yousuke Takahama (University of Tokushima) Cortex-medulla migration of thymocytes and central tolerance

10:30 - 11:00 Nikolaus Trede (University of Utah) In-vivo imaging in zebrafish &endash; a novel tool to tackle the genetics of autoimmunity


T lymphopoiesis, Chair: Juan Carlos Zúñiga-Pflücker

11:30 - 12:00 Irving Goldschneider (University of Connecticut) Prothymocyte development is defective in IL-7Ra-/-, but not IL-7-/-, mice

12:00 - 12:15 Harumi Suzuki (Yamaguchi University) Effect of LIF on T-poiesis from multipoteintial Pax5 deficient pro-B cells

12:15 - 12:30 Shinya Ogawa (Kyoto University) Regulation of IL-7 receptor expression during T cell development

12:30 - 12:45 Shinji Fujimoto (Kyoto University) Forced Id2 expression generates NK cells from fully specified T cell precursors in murine fetal thymus

12:45 - 13:00 Yasutoshi Agata (Kyoto University) E2A proteins induce chromatin accessibility for V(D)J recombination through the alteration of histone modification


Early thymocyte development, Chair: Eric Jenkinson

15:00 - 15:30 Robson MacDonald (Ludwig Institute for Cancer Research) Role of Notch and pre-TCR signaling in T cell development and lineage commitment

15:30 - 16:00 Juan Carlos Zúñiga-Pflücker (University of Toronto) The importance of Notch signals at the b-selection checkpoint

16:00 - 16:15 Masaki Miyazaki (Hiroshima University) Stability of Polycomb group protein influences intrathymic T cell development during DN to DP transition

16:15 - 16:30 Toshio Watanabe (Tohoku University) Forced expression of either one of two Runx1 isoforms exert differential effects on the thymocyte development


Lineage determination to T cells, Chair: Hiroshi Kawamoto

17:00 - 17:30 Eric Jenkinson (University of Birmingham) T/B lineage choice occurs prior to intrathymic Notch signaling

17:30 - 17:45 Katsuto Hozumi (Tokai University) Pre-thymic Notch signaling is necessary for GATA3 to work in the earliest stage of T cell development

17:45 - 18:00 Arivazhagan Sambandam (University of Pennsylvania) Notch signaling controls the generation and differentiation of early T lineage progenitors

18:00 - 18:30 Hiroshi Kawamoto (RCAI RIKEN) Macrophage potential retained by early thymic T cell progenitors


18:30 - 19:30 Posters with drink

P1 Michiyuki Kasai (NIID) A possible involvement of autophagy in presentation of cytoplasmic antigens via MHC class II molecules

P2 Junko Chiba (Nippon Veterinary and Animal Science University) Abnormal postnatal development of hypoplastic thymus in a new dwarf mutant rat strain PET

P3 Shizuko Kakinuma (NIRS) Cytokine production by thymic adherent cells during radiation-induced thymic lymphomagenesis

P4 Yi Shang (NIRS) Expression of cytokine receptors in radiation-induced mice thymic lymphoma

P5 Cunlan Liu (University of Tokushima) Differential roles of CCR7 and DOCK2 in cortex-medulla migration of positively selected thymocytes

P6 Hirofumi Shibata (Kyoto University) Regulation of IL-7 receptor expression by T cell receptor signal

P7 Atsushi Irie (Kumamoto University) PKD2, the major isoform of PKD family kinases expressed in the T cells and thymocytes, is involved in TCR-mediated T cell activation

P8 Jun Tsukada (RCAI RIKEN) Notch signals have a distinct role for naïve and memory CD4 T cells

P9 Hirotake Tsukamoto (Kumamoto University) TCR ligand avidity determines the mode of B-RAF/MEK/ERK activation leading to proliferative response in human CD4+ T cells

P10 Ryoji Yagi (RCAI RIKEN) CNS-2 is key element for IL-4 production in initial IL-4 producer cells

P11 Shinya Hirata (Kumamoto University) Prevention of experimental autoimmune encephalomyelitis via induction of T cells with regulatory function by transfer of ES-DC expressing MOG peptide along with TRAIL


19:30 Getting together


April 9

Transcriptional regulation of T cell differentiation, Chair: Ellen Rothenberg

9:00 - 9:30 Ellen Rothenberg (California Institute of Technology) Transcription factor interplay in T lineage commitment

9:30 - 10:00 Takehito Sato (Tokai University School of Medicine) Dual functions of Runx proteins for re-activating CD8 and silencing CD4 at the commitment process into CD8 thymocytes

10:00 - 10:15 Kazuyoshi Kohu (Tohoku University) Over-expression of the Runx3 transcription factor increases the proportion of mature thymocytes of the CD8 single positive lineage

10:15 - 10:30 Ichiro Taniuchi (RCAI RIKEN) Roles of Runx transcriptional factor complex during lymphocyte development


T cell selection, Chair: Sonoko Habu

11:15 - 11:45 Sho Yamasaki (RCAI RIKEN) Molecular mechanism of pre-TCR-mediated signal transduction

11:45 - 12:15 Osami Kanagawa (RCAI RIKEN) The ability of individual thymocytes to generate multiple VJ alpha rearrangements is critical for normal T cell development

12:15 - 12:45 Alfred Singer (National Cancer Institute) Cytokine regulation of coreceptor expression: Implications for T cell selection and reactivity

12:45 - 13:00 Keiko Udaka (Kochi Medical School) Raft-associated presentation of MHC class II molecules guides thymocytes to the CD4 lineage


Signals regulating T cell development and responses, Chair: Takashi Saito

15:00 - 15:30 Michael Dustin (New York University) Patterns of tyrosine phosphorylation in the immunological synapse

15:30 - 16:00 Satoshi Matsuda (Keio University) Spry1 functions as a negative-feedback loop in the TCR signaling pathway

16:00 - 16:30 Rose Zamoyska (National Institute for Medical Research) Contrasting roles for p56lckand p59fyn in regulating naïve T cell responses

16:30 - 16:45 Yoshinori Fukui (Kyushu University) DOCK2, a regulator of the actin cytoskeleton in lymphocytes, is a novel molecular target for controlling allo-graft rejection and autoimmune diseases

16:45 - 17:00 Hidehiro Kishimoto (Tokyo University of Science) Distinct function of NF-kB2-relB activation between semi-mature thymocytes and mature T cells


T cell recognition and responses, Chair: Shigeo Koyasu

17:30 - 18:00 Joost van Meerwijk (INSERM) Molecular signature of recent thymic selection events on effector and regulatory CD4+ T lymphocytes

18:00 - 18:30 Leonore Herzenberg (Stanford University) Culturing lymphocytes at atmospheric oxygen levels impacts T cell function

18:30 - 18:40 Kazuko Shibuya (University of Tsukuba) Requirements of the serine at residue 329 for lipid rafts recruitment of DNAM-1 (CD226)

18:40 - 18:50 Shuhei Tomita (University of Tokushima) Hypoxia inducible factor-1a regulates in vivo T cell responses

18:50 - 19:00 Takamasa Ueno (Kumamoto University) Functionally impaired HIV-specific CD8 T cells show high-affinity TCR-ligand interactions

19:00 - 19:10 Sachiko Kondo (Toyama Medical and Pharmaceutical University) Detection of antigen-specific lymphocytes with microwell-array system

19:10 - 19:20 Yoshimasa Tanaka (Kyoto University) Analysis of mechanism for nonpeptide antigen recognition by human gd T cells

19:20 - 19:30 Satoru Senju (Kumamoto University) Therapeutic control of immune responses by genetically modified ES cell-derived dendritic cells


April 10

Signals regulating helper T-cell differentiation, Chair: Toshinori Nakayama

8:30 - 9:00 Mark Bix (University of Washington) Chromosome specific analysis of cis-acting DNA regulatory elements on Il4 locus

9:00 - 9:30 Masakatsu Yamashita (Chiba University) Ras-Erk MAPK cascade, GATA3, and the ubiquitin-proteasome pathway in Th2 differentiation

9:30 - 9:45 Shinsuke Taki (Shinshu University) Homeostatic regulation of the Th1/Th2 balance by controlling basophil population size

9:45 - 10:00 Motoko Kimura (Chiba University) Regulation of Th2 cell differentiation by murine schnurri-2

10:00 - 10:15 Masato Kubo (RCAI RIKEN) Regulation of initial IL-4 production in memory like Th2 cells by RBP-J/Notch signaling through conserved non-cording sequence (CNS)-2 on IL-4 locus


T cell homeostasis and memory, Chair: Rose Zamoyska

10:45 - 11:15 Janko Nikolich-Zugich (Oregon Health & Science University) T-cell repertoire generation, diversity, maintenance and aging

11:15 - 11:30 Akemi Sakamoto (Chiba University) Bcl6 acts as an amplifier for the generation of central memory CD8 T cells

11:30 - 11:45 Shinya Tanaka (RCAI RIKEN) The physiological role of intrinsic memory-like T cells expressing IL-4 by primary antigen-stimulation

11:45 - 12:00 Hiroyuki Tezuka (Akita University) Impairment of APRIL-dependent IgA class-switch recombination in iNOS-/- mice

12:00 - 12:30 Elizabeth Simpson (Hammersmith Hospital) T cell responses to endogenous epitopes: balancing tolerance and immunity

Sandwich and coffee

Immune regulation and diseases, Chair:Nagahiro Minato

13:15 - 13:45 Shimon Sakaguchi (Kyoto University) Development of autoimmune arthritis due to a mutation of the ZAP-70 gene: T cell selection shift and altered Treg function

13:45 - 14:15 Bruno Kyewski (German Cancer Research Center) Promiscuous gene expression in thymic epithelial cells is regulated at multiple levels

14:15 - 14:45 Richard Boyd (Monash University) Restoration of thymic function and enhancement of haemopoiesis and lymphopoiesis following haemopoietic stem cell transplantation: re-shaping immunity for health

14:45 - 15:00 Wilfred Germeraad (University Hospital Maastricht) Tumour associated antigen mucin-1 is expressed on medullary epithelial cells in the thymus

15:00 - 15:15 Kenichiro Seino (Juntendo University) Immunoregulatory T cells and transplantation tolerance

15:15 - 15:45 Nagahiro Minato (Kyoto University) Chronic leukemia and systemic autoimmunity as hematopoietic stem cell disorders

Closing remarks



* Meeting organizers

Yousuke Takahama and Sonoko Habu

* Program Committee (JSPS Thymus)

Georg Hollander, Graham Anderson, Richard Boyd, Howard Petrie,
Hiroshi Kawamoto, Yousuke Takahama

* Program Committee (KTCC)

Sonoko Habu, Shigeo Koyasu, Nagahiro Minato, Toshinori Nakayama, Takashi Saito,
Shimon Sakaguchi, Yousuke Takahama

Last update April 22, 2005